GFP APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4240A
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212
Ser2-Lys238
Accession # P42212
Specificity
Detects GFP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GFP APC-conjugated Antibody
Detection of GFP in HEK293 Human Cell Line Transfected with GFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) wild-type GFP or (B) eGFP was stained with Goat Anti-GFP APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4240A). Quadrant markers were set based on control antibody staining (Catalog # IC108A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for GFP APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with wild-type GFP or eGFP was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HEK293 human embryonic kidney cell line transfected with wild-type GFP or eGFP was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GFP
Green Fluorescent Protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta-barrel with a chromophore (aa 65-67) containing alpha-helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.
Additional GFP Products
Product Documents for GFP APC-conjugated Antibody
Product Specific Notices for GFP APC-conjugated Antibody
For research use only
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