GIF Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90225

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-90225-100ul
NBP2-90225-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GIF Antibodies »

Product Summary for GIF Antibody (007)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human GIF (Uniprot#: P27352-1; Met1-Tyr417).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GIF Antibody (007)

Immunocytochemistry/ Immunofluorescence: GIF Antibody (007) [NBP2-90225]

Immunocytochemistry/ Immunofluorescence: GIF Antibody (007) [NBP2-90225]

Immunocytochemistry/Immunofluorescence: GIF Antibody (007) [NBP2-90225] - Immunofluorescence staining of GIF in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human GIF monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm .
Flow Cytometry: GIF Antibody (007) [NBP2-90225]

Flow Cytometry: GIF Antibody (007) [NBP2-90225]

Flow Cytometry: GIF Antibody (007) [NBP2-90225] - Flow cytometric analysis of Human GIF expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human GIF, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for GIF Antibody (007)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIF

GIF is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq]

Long Name

Gastric Intrinsic Factor

Alternate Names

IF, IFMH, INF, TCN3

Gene Symbol

CBLIF

Additional GIF Products

Product Documents for GIF Antibody (007)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GIF Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.