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GIF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81611

Novus Biologicals, part of Bio-Techne
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NBP1-81611

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAILALCQKNS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GIF Antibody

Western Blot: GIF Antibody [NBP1-81611]

Western Blot: GIF Antibody [NBP1-81611]

Western Blot: GIF Antibody [NBP1-81611] - Analysis in control (vector only transfected HEK293T lysate) and GIF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81611]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81611]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81611] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81611]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81611]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81611] - Staining of human liver shows low expression as expected.

Applications for GIF Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIF

GIF is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq]

Long Name

Gastric Intrinsic Factor

Alternate Names

IF, IFMH, INF, TCN3

Gene Symbol

CBLIF

Additional GIF Products

Product Documents for GIF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GIF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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