Gigaxonin Antibody

Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - U251 cells after 48hrs.

Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - Transfection with wild type Gigaxonin. Image from verified customer review.

Western Blot: Gigaxonin Antibody [NBP1-49924] -

Western Blot: Gigaxonin Antibody [NBP1-49924] - Design of proteomic bait to determine the binding partners of GIG.(A) GIG is a BTB/Kelch protein, & these domains are separated by an intervening BACK domain. GIG is thought to dimerize through its BTB domain [16]. The BTB domain has also been shown to bind to E3 ligases, potentially for ubiquitin tagging of Kelch targets. To identify potential substrates for degradation/modification (Kelch binding partners) we expressed flag-tagged full-length GIG (B) or its BTB domain (C). (D) Sequence of proteomic baits with key regions identified by color. The constructs expressed included a single flag tag & linker (grey), Biotag (black), BTB domain (blue), BACK domain (red), & Kelch domains (green). The location of the truncation for the BTB bait shown in (C) is indicated by the black line at amino acid 268, towards the N-terminus of the BACK domain. (E) Flag-tagged constructs were expressed in HEK293 cells & affinity purified with an anti-Flag antibody (abbreviated as F). The membrane was then probed with anti-Flag tag antibodies. Untransfected cells (NC) were included to control for non-specific binding to the antibody or beads. Flag-tagged GIG was ~62 kDa, while the BTB domain was ~34 kDa & forms a dimer in the immunoprecipitation sample that was ~70 kDa. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26460568), licensed under a CC-BY license. Not internally tested by Novus Biologicals.