Skip to main content

GIPC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1353

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB100-1353

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-GAIGDAKVGRY corresponding to C-Terminus according to NP_974197.1, NP_974199.1, NP_974196.1, NP_974198.1, NP_974223.1.

Localization

Cytoplasmic and Plasma membrane

Specificity

This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for GIPC1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.035ug/ml
Application Notes
WB: Approx. 38 kDa band and strong 23-24 kDa band bands observed in lysates of HeLa, and approx. 24 kDa band also observed in human pancreas, kidney and muscle lysates (calculated MW of 36.0 kDa band according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1 kDa band according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GIPC1

GIPC1, GIPC2, and GIPC3 are a family of central PDZ-domain proteins with GH1 and GH2 domains. GIPC1 has been shown to be highly expressed in gastric, pancreatic, colorectal and lung cancer cell lines. It also has a list of identified binding partners in vitro including many transmembrane proteins and cell surface molecules involved in adhesion. In vivo, there is evidence that suggests a role of GIPC1 in transmembrane protein trafficking through the Golgi stacks and possibly a role in the later stages of vesicle trafficking.

Alternate Names

C19orf3chromosome 19 open reading frame 3, GAIP C-terminus-interacting protein, GIPC PDZ domain containing family, member 1, GIPCRGS-GAIP-interacting protein, GLUT1 C-terminal binding protein, GLUT1CBP, IGF-1 receptor interacting protein 1, MGC15889, MGC3774, NIP, PDZ domain-containing protein GIPC1, regulator of G-protein signalling 19 interacting protein 1, RGS19IP1IIP-1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP-2RGS19-interacting protein 1

Entrez Gene IDs

10755 (Human); 67903 (Mouse); 83823 (Rat)

Gene Symbol

GIPC1

UniProt

Additional GIPC1 Products

Product Documents for GIPC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GIPC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...