GIT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16661

Novus Biologicals, part of Bio-Techne
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NBP2-16661
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Chicken (96%), Rat (97%), Rhesus Macaque (99%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GIT2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GIT2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GIT2 Antibody

Western Blot: GIT2 Antibody [NBP2-16661]

Western Blot: GIT2 Antibody [NBP2-16661]

Western Blot: GIT2 Antibody [NBP2-16661] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GIT2 Antibody [NBP2-16661]

Immunocytochemistry/ Immunofluorescence: GIT2 Antibody [NBP2-16661]

Immunocytochemistry/Immunofluorescence: GIT2 Antibody [NBP2-16661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GIT2 protein stained by GIT2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Frozen: GIT2 Antibody [NBP2-16661]

Immunohistochemistry-Frozen: GIT2 Antibody [NBP2-16661]

Immunohistochemistry-Frozen: GIT2 Antibody [NBP2-16661] - GIT2 antibody detects GIT2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: GIT2 protein stained by GIT2 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
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Applications for GIT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GIT2

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq]

Alternate Names

ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT2, CAT-2, cool-associated, tyrosine phosphorylated protein 2, Cool-interacting tyrosine-phosphorylated protein 2, DKFZp686G01261, G protein-coupled receptor kinase interacting ArfGAP 2, G protein-coupled receptor kinase interactor 2, G protein-coupled receptor kinase-interactor 2, KIAA0148GRK-interacting protein 2, MGC760

Gene Symbol

GIT2

UniProt

Q14161 (Human)

Additional GIT2 Products

Product Documents for GIT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GIT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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