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GIT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13188

Novus Biologicals, part of Bio-Techne
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NBP3-13188

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse GIT2. The exact sequence is proprietary.

Reactivity Notes

Human (85%), Rhesus Monkey (85%), Bovine (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GIT2 Antibody

Western Blot: GIT2 Antibody [NBP3-13188]

Western Blot: GIT2 Antibody [NBP3-13188]

Western Blot: GIT2 Antibody [NBP3-13188] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: GIT2 Antibody [NBP3-13188]

Western Blot: GIT2 Antibody [NBP3-13188]

Western Blot: GIT2 Antibody [NBP3-13188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:1000.
Western Blot: GIT2 Antibody [NBP3-13188]

Western Blot: GIT2 Antibody [NBP3-13188]

Western Blot: GIT2 Antibody [NBP3-13188] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:1000.

Applications for GIT2 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIT2

GIT2 encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Alternate Names

ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT2, CAT-2, cool-associated, tyrosine phosphorylated protein 2, Cool-interacting tyrosine-phosphorylated protein 2, DKFZp686G01261, G protein-coupled receptor kinase interacting ArfGAP 2, G protein-coupled receptor kinase interactor 2, G protein-coupled receptor kinase-interactor 2, KIAA0148GRK-interacting protein 2, MGC760

Gene Symbol

GIT2

Additional GIT2 Products

Product Documents for GIT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GIT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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