GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81089
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free [NBP2-81089]](https://resources.bio-techne.com/images/products/GITR-Ligand-TNFSF18-Antibody-YGL386-Immunocytochemistry-Immunofluorescence-NBP2-81089-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free [NBP2-81089]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # YGL386
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein injected into DA rats.
Specificity
This antibody binds murine GITR Ligand/TNFSF18.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free [NBP2-81089]
Immunocytochemistry/Immunofluorescence: GITR Ligand/TNFSF18 Antibody (YGL386) [NBP2-81089] - Immunofluorescence staining of fixed mouse splenocytes with GITR Ligand/TNFSF18 Antibody (YGL386) [NBP2-81089]. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YGL386 [NBP2-81089] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81089, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.Applications for GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to GITRL and was used in the identification of GITRL and confirmation of its function as the ligand for GITR.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: GITR Ligand/TNFSF18
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene Ligand
Alternate Names
AITRL, GITRL, TNFSF18
Gene Symbol
TNFSF18
Additional GITR Ligand/TNFSF18 Products
Product Documents for GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free
Product Specific Notices for GITR Ligand/TNFSF18 Antibody (YGL386) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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