GLI-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29457

Novus Biologicals, part of Bio-Techne
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NBP3-29457
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GLI-2 Antibodies »

Product Specifications

Immunogen

Between 950 and 1000

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-2 Antibody - BSA Free

GLI-2 Antibody

Immunoprecipitation:GLI-2 AntibodyNBP3-29457] -

Immunoprecipitation:GLI-2 AntibodyNBP3-29457] -Detection of human GLI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLI2 antibody NBP3-29457 (lot NBP3-29457-1) used for IP at 6 µg per reaction. GLI2 was also immunoprecipitated by rabbit anti-GLI2 antibodies BL20733 and BL20735. For blotting immunoprecipitated GLI2, NBP3-29457 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for GLI-2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GLI-2

Gli2 belongs to the C2H2 type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes Greig cephalopolysyndactyly syndrome, Pallister Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Long Name

GLI-Kruppel family member GLI2

Alternate Names

GLI2, THP2

Gene Symbol

GLI2

Additional GLI-2 Products

Product Documents for GLI-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GLI-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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