GLIS3 Antibody (PCRP-GLIS3-1B11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13869

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PCRP-GLIS3-1B11

Concentration

0.2 mg/ml

View all GLIS3 Antibodies »

Product Summary for GLIS3 Antibody (PCRP-GLIS3-1B11)

Immunogen

Recombinant full-length human GLIS3 protein (Uniprot: Q8NEA6)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14052)

Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for GLIS3 Antibody (PCRP-GLIS3-1B11)

Flow Cytometry: GLIS3 Antibody (PCRP-GLIS3-1B11) [NBP3-13869]

Flow Cytometry: GLIS3 Antibody (PCRP-GLIS3-1B11) [NBP3-13869]

Flow Cytometry: GLIS3 Antibody (PCRP-GLIS3-1B11) [NBP3-13869] - Flow cytometric analysis of PFA-fixed HeLa cells. GLIS3 antibody (PCRP-GLIS3-1B11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: GLIS3 Antibody (PCRP-GLIS3-1B11) [NBP3-13869]

Protein Array: GLIS3 Antibody (PCRP-GLIS3-1B11) [NBP3-13869]

Protein Array: GLIS3 Antibody (PCRP-GLIS3-1B11) [NBP3-13869] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GLIS3-Monospecific Mouse Monoclonal Antibody (PCRP-GLIS3-1B11).

Applications for GLIS3 Antibody (PCRP-GLIS3-1B11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GLIS3

The GLIS3 gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-typezinc finger domains. This protein functions as both a repressor and activator of transcription and is specificallyinvolved in the developm

Alternate Names

FLJ38999, FLJ90578, GLIS family zinc finger 3, GLI-similar 3, OK/KNS-cl.4, zinc finger protein 515, zinc finger protein GLIS3

Gene Symbol

GLIS3

UniProt

Q8NEA6 (Human)

Additional GLIS3 Products

Product Documents for GLIS3 Antibody (PCRP-GLIS3-1B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GLIS3 Antibody (PCRP-GLIS3-1B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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