GLP-1R Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NLS1205
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Simple Western, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an N-terminal extracellular portion of the human GLP1R protein (between residues 100-200). [UniProt P43220]
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 26398375).
Localization
Cell membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GLP-1R Antibody - BSA Free
Simple Western: GLP-1R AntibodyBSA Free [NLS1205]
Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.Immunocytochemistry: GLP-1R Antibody - BSA Free [NLS1205]
Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) GLP-1 receptors are labelled in red. (k) DAPI staining indicates the cell nucleus (blue). (l) Merged images. (m-p) Evaluation of GLP-1 receptors expression in human retinal pericytes (HRPs). (m) Pericytes labelled with alpha-SMA antibodies (green). (n) GLP-1 receptors are labelled in red. (o) DAPI staining indicates the cell nucleus (blue). (l and p) merged images. Image collected and cropped by CiteAb from the following publication (https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.Western Blot: GLP-1R AntibodyBSA Free [NLS1205]
Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.Applications for GLP-1R Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 32232832)
Immunohistochemistry
10 - 20 ug/mL
Immunohistochemistry-Paraffin
10 - 20 ug/mL
Microarray
reported by customer review
Simple Western
1:50
Western Blot
1 - 2 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NLS1205 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: GLP-1R
Long Name
Glucagon-like Peptide 1 Receptor
Alternate Names
GLP1R
Entrez Gene IDs
2740 (Human)
Gene Symbol
GLP1R
UniProt
Additional GLP-1R Products
Product Documents for GLP-1R Antibody - BSA Free
Product Specific Notices for GLP-1R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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