GLS2 Antibody (CL7716)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88907

Novus Biologicals, part of Bio-Techne
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NBP2-88907
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL7716

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GLS2 Antibodies »

Product Summary for GLS2 Antibody (CL7716)

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: GRETPHSHQPQHQDHDSSE

Reactivity Notes

Mouse, Rat (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GLS2 Antibody (CL7716)

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907]

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907]

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Analysis in human liver and skeletal muscle tissues using NBP2-88907 antibody. Corresponding GLS2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907]

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907]

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907]

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907]

Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
View 2 more images

Applications for GLS2 Antibody (CL7716)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLS2

The protein encoded by the GLS2 gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. At least one transcribed pseudogene has been found for this gene. [provided by RefSeq]

Alternate Names

EC 3.5.1.2, GAbreast cell glutaminase, GLSglutaminase GA, glutaminase 2 (liver, mitochondrial), glutaminase I, glutaminase liver isoform, mitochondrial, hLGA, LGA, L-glutaminase, L-glutamine amidohydrolase, MGC71567, phosphate-activated glutaminase, phosphate-dependent glutaminase, truncated glutaminase 2

Gene Symbol

GLS2

Additional GLS2 Products

Product Documents for GLS2 Antibody (CL7716)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GLS2 Antibody (CL7716)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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