Key Product Details

Validated by

Biological Validation

Species Reactivity

Epitope Tag

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Glu-Glu Epitope Tag Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with glu-glu cleavage site (EYMPME) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Rabbit anti- human glu-glu affinity purified antibodies were coupled to peptide immobilized on solid support.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glu-Glu Epitope Tag Antibody - BSA Free

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-354]

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-354]

Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).
Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-354]

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-354]

Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).
Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-354]

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-354]

Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Triton X-100(1%0 Whole cell lysate(20mcg) from Cos7 cells transiently transfected with control vector (v) or an expression vector driving expression of Glu-Glu tagged RalBP1 (a to d).

Applications for Glu-Glu Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

Primary-1:1000-1:30000; Coating: 1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000-1:30000
Application Notes
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cyto 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.

Reviewed Applications

Read 1 review rated 1 using NB600-354 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Glu-Glu Epitope Tag

Alternate Names

EYMPME epitope tag, EYMPME tag, Glu-Glu epitope tag

Additional Glu-Glu Epitope Tag Products

Product Documents for Glu-Glu Epitope Tag Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glu-Glu Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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