Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Glucokinase/GCK Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GCK (NP_000153) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free

Western Blot: Glucokinase/GCK Antibody (OTI3E3)Azide and BSA Free [NBP2-70822]

Western Blot: Glucokinase/GCK Antibody (OTI3E3)Azide and BSA Free [NBP2-70822]

Western Blot: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK.
Immunocytochemistry/ Immunofluorescence: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822]

Immunocytochemistry/ Immunofluorescence: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822]

Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK.
Immunohistochemistry: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822]

Immunohistochemistry: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822]

Immunohistochemistry: Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free [NBP2-70822] - Staining of paraffin-embedded Human breast tissue using anti-GCK mouse monoclonal antibody.
View 8 more images

Applications for Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucokinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose-6-phosphate, GCK has much lower affinity for glucose and is not inhibited by the product. Consequently, GCK has a Km for glucose of approximately 7 mM, which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non-insulin-dependent diabetes mellitus, maturity-onset diabetes of the young type 2, and hyperinsulinemia of infancy. The enzyme activity was measured using a phosphatase coupled kinase assay.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Gene Symbol

GCK

Additional Glucokinase/GCK Products

Product Documents for Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucokinase/GCK Antibody (OTI3E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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