Glucose 6 Phosphate Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60954

Novus Biologicals, part of Bio-Techne
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NB100-60954
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all G6PD Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 ; NP_001035810.1.

Epitope

STNSDDVRDEKVK

Specificity

This antibody is expected to recognize both reported isoforms (NP_000393.4 and NP_001035810.1).

Marker

Cytosol Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Analysis of Human Testis lysate (35ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Staining of paraffin embedded Human Spleen. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Staining of paraffin embedded Human Testis. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Glucose 6 Phosphate Dehydrogenase Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
WB: Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Primary incubation was 1 hour.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: G6PD

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Glucose-6-Phosphate Dehydrogenase

Alternate Names

G6PDH

Entrez Gene IDs

2539 (Human); 14381 (Mouse); 24377 (Rat)

Gene Symbol

G6PD

UniProt

NP_000393.4 (Human)

Additional G6PD Products

Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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