Glucose 6 Phosphate Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89805

Novus Biologicals, part of Bio-Techne
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NBP1-89805
NBP1-89805-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all G6PD Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKP

Marker

Cytosol Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis using Anti-G6PD antibody NBP1-89805 (A) shows similar pattern to independent antibody NBP1-89804 (B).
Immunocytochemistry/ Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805]

Immunocytochemistry/ Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805]

Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
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Applications for Glucose 6 Phosphate Dehydrogenase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G6PD

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Long Name

Glucose-6-Phosphate Dehydrogenase

Alternate Names

G6PDH

Gene Symbol

G6PD

Additional G6PD Products

Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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