Glucose 6 phosphate isomerase Antibody (12A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26548

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26548-100ul
NBP3-26548-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 12A4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glucose 6 phosphate isomerase Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Glucose 6 phosphate isomerase [UniProt P06744]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glucose 6 phosphate isomerase Antibody (12A4)

Glucose 6 phosphate isomerase Antibody (12A4)

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Image of Glucose 6 phosphate isomerase Antibody (12A4) diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.45% DAB.
Glucose 6 phosphate isomerase Antibody (12A4)

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Image of Glucose 6 phosphate isomerase Antibody (12A4) diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.45% DAB.
Glucose 6 phosphate isomerase Antibody (12A4)

Western Blot: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Western Blot: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Positive Western Blot detected in: A549 whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, HELA whole cell lysate, PC-3 whole cell lysate.
All lanes: Glucose 6 phosphate isomerase Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 63 kDa
Observed band size: 63 kDa
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Applications for Glucose 6 phosphate isomerase Antibody (12A4)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Glucose 6 phosphate isomerase

Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Alternate Names

AMFGNPI, Autocrine motility factor, DKFZp686C13233, EC 5.3.1.9, glucose phosphate isomerase, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, Neuroleukin, NLKSA36, oxoisomerase, PGI, PHI, Phosphoglucose isomerase, phosphohexomutase, Phosphohexose isomerase, phosphosaccharomutase, SA-36, Sperm antigen 36, sperm antigen-36

Gene Symbol

GPI

Additional Glucose 6 phosphate isomerase Products

Product Documents for Glucose 6 phosphate isomerase Antibody (12A4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glucose 6 phosphate isomerase Antibody (12A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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