Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Glucosylceramidase/GBA Antibodies »

Product Summary for Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 40-315 of human GBA (NP_000148) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

55.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

Western Blot: Glucosylceramidase/GBA Antibody (OTI4G4)Azide and BSA Free [NBP2-70803]

Western Blot: Glucosylceramidase/GBA Antibody (OTI4G4)Azide and BSA Free [NBP2-70803]

Western Blot: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA .
Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803]

Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803]

Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803]

Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803]

Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucosylceramidase/GBA

GBA encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein.

Alternate Names

Alglucerase, GBA, GBA1, Imiglucerase

Gene Symbol

GBA1

Additional Glucosylceramidase/GBA Products

Product Documents for Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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