Glut1 Antibody (GLUT1/2476) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75787
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # GLUT1/2476
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human Glut1 protein (around aa 203-305) (exact sequence is proprietary) (Uniprot: P11166)
Localization
Cell Surface
Marker
Tumor Progression and Mesothelioma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75785).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Glut1 Antibody (GLUT1/2476) - Azide and BSA Free
Western Blot: Glut1 Antibody (GLUT1/2476)Azide and BSA Free [NBP2-75787]
Western Blot: Glut1 Antibody (GLUT1/2476) - Azide and BSA Free [NBP2-75787] - Western blot analysis of human A431 cell lysate using Glut1 antibody (GLUT1/2476).Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) - Azide and BSA Free [NBP2-75787]
Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) - Azide and BSA Free [NBP2-75787] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476).Flow Cytometry: Glut1 Antibody (GLUT1/2476) - Azide and BSA Free [NBP2-75787]
Flow Cytometry: Glut1 Antibody (GLUT1/2476) - Azide and BSA Free [NBP2-75787] - Flow cytometric analysis of K562 cells using Glut1 antibody (GLUT1/2476) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for Glut1 Antibody (GLUT1/2476) - Azide and BSA Free
Application
Recommended Usage
ELISA
1 - 2 ug/mL
Immunohistochemistry-Paraffin
1 - 2 ug/mL
Application Notes
ELISA: For coating use Ab at 1-2ug/ml, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Glut1
GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).
References
1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315
2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001
3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6
4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7
5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17
6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023
Long Name
Glucose Transporter Type 1
Alternate Names
DYT17, DYT18, DYT9, EIG12, GLUT1DS, SLC2A1
Gene Symbol
SLC2A1
UniProt
Additional Glut1 Products
Product Documents for Glut1 Antibody (GLUT1/2476) - Azide and BSA Free
Product Specific Notices for Glut1 Antibody (GLUT1/2476) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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