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Glut5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45227

Novus Biologicals, part of Bio-Techne
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NBP1-45227

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SEVYPEKEELKE corresponding to C-Terminus according to NP_003030.1.

Epitope

C-Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glut5 Antibody

Immunohistochemistry-Paraffin: Glut5 Antibody [NBP1-45227]

Immunohistochemistry-Paraffin: Glut5 Antibody [NBP1-45227]

Immunohistochemistry-Paraffin: Glut5 Antibody [NBP1-45227] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Glut5 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 38kDa band in Human Duodenum, Kidney and Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.0kDa according to NP_003030.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Small Intestin

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut5

Facilitative simple sugar uptake by mammalian cells is mediated by a family of glucose transporters. Gluts are predicted to contain 12 transmembrane domains with both amino- and carboxyl-termini located within the cytosol. The individual Gluts differ in their tissue distribution, substrate specificity, kinetic properties, and intracellular localization. These functional differences allow members of the Glut family to finely regulate whole-body glucose homeostasis.

Long Name

Glucose Transporter Type 5

Alternate Names

SLC2A5

Entrez Gene IDs

6518 (Human)

Gene Symbol

SLC2A5

Additional Glut5 Products

Product Documents for Glut5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glut5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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