Glutamate Dehydrogenase Antibody (SR1881)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22240

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22240-100ul
NBP3-22240-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1881

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glutamate Dehydrogenase 1/GLUD1 Antibodies »

Product Summary for Glutamate Dehydrogenase Antibody (SR1881)

Immunogen

A synthesized peptide derived from human Glutamate Dehydrogenase (Uniprot #: P00367)

Specificity

Detects endogenous levels of total Glutamate Dehydrogenase

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamate Dehydrogenase Antibody (SR1881)

Western Blot: Glutamate Dehydrogenase Antibody (SR1881) [NBP3-22240] -

Western Blot: Glutamate Dehydrogenase Antibody (SR1881) [NBP3-22240] - Western blot analysis using NBP3-22240 in HepG2 cell lysate.

Applications for Glutamate Dehydrogenase Antibody (SR1881)

Application
Recommended Usage

Flow Cytometry

1:500

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 1/GLUD1

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Alternate Names

GDH, GDH1

Gene Symbol

GLUD1

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Antibody (SR1881)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutamate Dehydrogenase Antibody (SR1881)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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