Glutamate Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16679

Novus Biologicals, part of Bio-Techne
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NBP2-16679
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Cited:

Human, Mouse

Predicted:

Bovine (98%), Porcine (99%), Sheep (99%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Glutamate Dehydrogenase 1/GLUD1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%).

Localization

Mitochondrion matrix

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamate Dehydrogenase Antibody

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679]

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679]

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***P<0.001. Data were expressed as mean +/- SEM. Three replicated experiments were carried out with the similar results. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Rat liver. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for Glutamate Dehydrogenase Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 1/GLUD1

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]

Alternate Names

GDH, GDH1

Gene Symbol

GLUD1

UniProt

P00367 (Human)

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutamate Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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