Glutamate Receptor 6 Antibody (JE39-33)

Western Blot: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405]

Western Blot: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405] - Western blot analysis of Glutamate Receptor 6 on different lysates with Rabbit anti-Glutamate Receptor 6 antibody (NBP3-32405) at 1/5,000 dilution.

Lane 1: Mouse brain tissue lysate (10 ug/Lane)
Lane 2: Mouse brain tissue lysate (70 heat) (10 ug/Lane)
Lane 3: Rat brain tissue lysate (10 ug/Lane)

Predicted band size: 103 kDa
Observed band size: 103 kDa

Exposure time: 53 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32405) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405]

Immunohistochemistry: Glutamate Receptor 6 Antibody (JE39-33) [NBP3-32405] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Glutamate Receptor 6 antibody (NBP3-32405) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32405) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.