I was wondering what the specificity of this enzyme is in regards to the glutaminase isoforms gls1 and gls2. Ideally, this would have no cross reactivity with gls2.

Name: Glutaminase Antibody (3211)
Brand: Novus Biologicals
SKU: NBP2-43762

Our Glutaminase antibody NBP2-43762 was made to an immunogen that falls between aa 551-669 of the human glutaminase C (GAC) protein: http://www.uniprot.org/uniprot/O94925#sequences. This antibody has been found to not cross react against the canonical (KGA) sequence for GLS1. It has not yet been tested for cross reactivity against the GLS2 protein. Based on an alignment of the immunogen range and the GLS2 sequence, there does seem to be some areas with significant homology, so there is a chance it could react.

Glutaminase Antibody (3211)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43762

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat, Porcine, Bovine, Ovine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 3211

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Glutaminase Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase (GAC). The exact sequence is proprietary.

Specificity

Specific for GAC but not KGA isoform.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutaminase Antibody (3211)

Western Blot: Glutaminase Antibody (3211) [NBP2-43762]

Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [3211] diluted at a dilution of 1:1000.

Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762]

Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [3211] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762]

Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762] - Mouse liver. Glutaminase C antibody [3211] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for Glutaminase Antibody (3211)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutaminase

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Gene Symbol

GLS

Additional Glutaminase Products

Product Documents for Glutaminase Antibody (3211)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Glutaminase Antibody (3211)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Glutaminase Antibody (3211)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43762

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Glutaminase Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for Glutaminase Antibody (3211)

Western Blot: Glutaminase Antibody (3211) [NBP2-43762]

Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762]

Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762]

Applications for Glutaminase Antibody (3211)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Glutaminase

Long Name

Alternate Names

Gene Symbol

Additional Glutaminase Products

Product Documents for Glutaminase Antibody (3211)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Glutaminase Antibody (3211)