Glutaminase Antibody (5C4)
Novus Biologicals, part of Bio-Techne | Catalog # H00002744-M01
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5C4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GLS (NP_055720, 580 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
Specificity
GLS - glutaminase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Glutaminase Antibody (5C4)
![Western Blot: Glutaminase Antibody (5C4) [H00002744-M01]](https://resources.bio-techne.com/images/products/Glutaminase-Antibody-5C4-Western-Blot-H00002744-M01-img0001.jpg "Western Blot: Glutaminase Antibody (5C4) [H00002744-M01]")
Western Blot: Glutaminase Antibody (5C4) [H00002744-M01]
Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).![Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01]](https://resources.bio-techne.com/images/products/Glutaminase-Antibody-5C4-Immunocytochemistry-Immunofluorescence-H00002744-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01]")
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01]
Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01] - Analysis of monoclonal antibody to GLS on HeLa cell. Antibody concentration 10 ug/ml.![ELISA: Glutaminase Antibody (5C4) [H00002744-M01]](https://resources.bio-techne.com/images/products/Glutaminase-Antibody-5C4-ELISA-H00002744-M01-img0006.jpg "ELISA: Glutaminase Antibody (5C4) [H00002744-M01]")
ELISA: Glutaminase Antibody (5C4) [H00002744-M01]
ELISA: Glutaminase Antibody (5C4) [H00002744-M01] - Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.Applications for Glutaminase Antibody (5C4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Glutaminase
Long Name
Glutaminase Kidney Isoform, Mitochondrial
Alternate Names
GLS, K-Glutaminase, L-Glutamine Amidohydrolase
Entrez Gene IDs
2744 (Human)
Gene Symbol
GLS
OMIM
138280 (Human)
UniProt
Additional Glutaminase Products
Product Documents for Glutaminase Antibody (5C4)
Product Specific Notices for Glutaminase Antibody (5C4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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