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Glutaminase Antibody (GT1075)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13512

Novus Biologicals, part of Bio-Techne
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NBP3-13512

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT1075

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Glutaminase Antibody (GT1075)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase (GAC). The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Glutaminase Antibody (GT1075)

Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512]

Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512]

Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase (GAC) antibody detects Glutaminase (GAC) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase (GAC) antibody (NBP3-13512) diluted at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (GT1075) [NBP3-13512]

Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (GT1075) [NBP3-13512]

Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase antibody [GT1075] detects Glutaminase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase protein stained by Glutaminase antibody [GT1075] (NBP3-13512) diluted at 1:400. Blue: Hoechst 33342 staining.

Applications for Glutaminase Antibody (GT1075)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutaminase

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Gene Symbol

GLS

Additional Glutaminase Products

Product Documents for Glutaminase Antibody (GT1075)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutaminase Antibody (GT1075)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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