Glutamine Synthetase Antibody (A3G2-R)

Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387]

Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: SK-Br-3 cell lysate
Lane 8: Human liver tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387]

Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32387) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.