Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07344
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344]](https://resources.bio-techne.com/images/products/Glutathione-Peroxidase-4-GPX4-Antibody-LHM-2-Immunocytochemistry-Immunofluorescence-NBP3-07344-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LHM 2
Concentration
0.2 mg/ml
Product Summary for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)
Immunogen
A 375P cells crude extract
Localization
Mitochondrion, Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08253)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344]
Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344] - Confocal immunofluorescence image of HepG2 cells stained with Glutathione Peroxidase 4/GPX4 Mouse Monoclonal Antibody (LHM 2) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuclei red.Applications for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Glutathione Peroxidase 4/GPX4
Additional Glutathione Peroxidase 4/GPX4 Products
Product Documents for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)
Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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