Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c
Novus Biologicals, part of Bio-Techne | Catalog # NB100-41379
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EEPLVIEKDLPHY corresponding to C-Terminus according to NP_002076.2, NP_001034937.1.
Specificity
This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c
![Western Blot: Glutathione Peroxidase 4/GPX4 AntibodyIsoform a and c [NB100-41379]](https://resources.bio-techne.com/images/products/Glutathione-Peroxidase-4-Isoform-a-and-c-Antibody-Western-Blot-NB100-41379-img0007.jpg "Western Blot: Glutathione Peroxidase 4/GPX4 AntibodyIsoform a and c [NB100-41379]")
Western Blot: Glutathione Peroxidase 4/GPX4 AntibodyIsoform a and c [NB100-41379]
Western Blot: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - Analysis of Glutathione Peroxidase 4 (Isoform a and c) in Human Testis lysate using this antibody at 0.1ug/ml.![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c [NB100-41379]](https://resources.bio-techne.com/images/products/Glutathione-Peroxidase-4-Isoform-a-and-c-Antibody-Immunohistochemistry-Paraffin-NB100-41379-img0006.jpg "Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c [NB100-41379]")
Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c [NB100-41379]
Immunohistochemistry-Paraffin: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - IHC analysis of Glutathione Peroxidase 4 (Isoform a and c) in paraffin embedded Human Testis using this antibody at 0.3 ug/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c
Application
Recommended Usage
Immunohistochemistry
0.3 ug/ml
Immunohistochemistry-Paraffin
0.3 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 19 kDa band observed in human kidney and testis lysates and in HEK293 lysates (calculated MW of 22.1 kDa band according to NP_002076.2). IHC-P: Human testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Glutathione Peroxidase 4/GPX4
Additional Glutathione Peroxidase 4/GPX4 Products
Product Documents for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c
Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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