Skip to main content

Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41379

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB100-41379

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-EEPLVIEKDLPHY corresponding to C-Terminus according to NP_002076.2, NP_001034937.1.

Specificity

This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

Western Blot: Glutathione Peroxidase 4/GPX4 AntibodyIsoform a and c [NB100-41379]

Western Blot: Glutathione Peroxidase 4/GPX4 AntibodyIsoform a and c [NB100-41379]

Western Blot: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - Analysis of Glutathione Peroxidase 4 (Isoform a and c) in Human Testis lysate using this antibody at 0.1ug/ml.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c [NB100-41379]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c [NB100-41379]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - IHC analysis of Glutathione Peroxidase 4 (Isoform a and c) in paraffin embedded Human Testis using this antibody at 0.3 ug/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

Application
Recommended Usage

Immunohistochemistry

0.3 ug/ml

Immunohistochemistry-Paraffin

0.3 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 19 kDa band observed in human kidney and testis lysates and in HEK293 lysates (calculated MW of 22.1 kDa band according to NP_002076.2). IHC-P: Human testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 4/GPX4

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Alternate Names

GPX4, PHGPx, snGPx

Entrez Gene IDs

2879 (Human)

Gene Symbol

GPX4

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...