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Glutathione S-Transferase/GST Antibody (GST13) - VHH

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12166

Novus Biologicals, part of Bio-Techne
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NBP3-12166

Key Product Details

Species Reactivity

E. coli

Applications

Dot Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Llama Clone # GST13

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Glutathione S-Transferase/GST

Specificity

Expect reactivity against GST-labeled probes and proteins.

Clonality

Monoclonal

Host

Llama

Description

This recombinant antibody clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture (E. coli) .

Store at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use

Scientific Data Images for Glutathione S-Transferase/GST Antibody (GST13) - VHH

Western Blot: Glutathione S-Transferase/GST Antibody (GST13)VHH [NBP3-12166]

Western Blot: Glutathione S-Transferase/GST Antibody (GST13)VHH [NBP3-12166]

Western Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Recombinant GST (0.2ug) [+]. Lane 3: HEK293T WCL / rec. GST(10ug/0.2ug) [+]. Lane 4: HEK293T Whole Cell Lysate (10ug) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (10ug) [-]. Primary Antibody: Anti-GST (VhH) at 5.0ug/mL overnight at 2-8 degrees C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:3,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 60 sec. Predicted MW: ~25.5kDa. Observed MW: ~25.5kDa. Unspecific Bands: ~70kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.
Dot Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Row A: Recombinant GST protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.5hr at RT. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:500 for 30 mins at RT. Block: BlockOut Buffer.

Applications for Glutathione S-Transferase/GST Antibody (GST13) - VHH

Application
Recommended Usage

Dot Blot

1 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:200
Application Notes
This product is a his-tagged monoclonal recombinant antibody designed to detect Glutathione-S-Transferase and its conjugates. This antibody has been tested by western blot and dot blot and is intended for use in immunological assays including western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-GST can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of GST-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS, pH 7.42, 20% (v/v) Glycerol

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione S-Transferase/GST

For the highly sensitive and specific detection of GST-tagged recombinant proteins in Western blots and ELISAs. In an indirect chemiluminescence system this product is quality tested todetect 10 ng of purified GST-tagged ubiquitin (N-terminal tag).

Alternate Names

Glutathione STransferase

Additional Glutathione S-Transferase/GST Products

Product Documents for Glutathione S-Transferase/GST Antibody (GST13) - VHH

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione S-Transferase/GST Antibody (GST13) - VHH

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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