Glutathione Synthetase Antibody (2S9W7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15402
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2S9W7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Glutathione Synthetase Antibody (2S9W7)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Glutathione Synthetase (GSS) (P48637). AIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Glutathione Synthetase Antibody (2S9W7)
![Western Blot: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402]](https://resources.bio-techne.com/images/products/Glutathione-Synthetase-Antibody-2S9W7-Western-Blot-NBP3-15402-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402]")
Western Blot: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402]
Western Blot: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402] - Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) Rabbit mAb (NBP3-15402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402]](https://resources.bio-techne.com/images/products/Glutathione-Synthetase-Antibody-2S9W7-Immunocytochemistry-Immunofluorescence-NBP3-15402-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402]")
Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402]
Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402] - Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) Rabbit mAb (NBP3-15402) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Glutathione Synthetase Antibody (2S9W7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Glutathione Synthetase
Alternate Names
EC 6.3.2.3, Glutathione synthase, glutathione synthetase, GSH synthetase, GSHS, GSH-S, MGC14098
Gene Symbol
GSS
Additional Glutathione Synthetase Products
Product Documents for Glutathione Synthetase Antibody (2S9W7)
Product Specific Notices for Glutathione Synthetase Antibody (2S9W7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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