Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70854

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Glutathione Synthetase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

Western Blot: Glutathione Synthetase Antibody (OTI1B8)Azide and BSA Free [NBP2-70854]

Western Blot: Glutathione Synthetase Antibody (OTI1B8)Azide and BSA Free [NBP2-70854]

Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854]

Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854]

Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854]

Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854]

Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.
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Applications for Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Synthetase

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage byfree radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as ahomodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion ofgamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.(provided by RefSeq)

Alternate Names

EC 6.3.2.3, Glutathione synthase, glutathione synthetase, GSH synthetase, GSHS, GSH-S, MGC14098

Gene Symbol

GSS

Additional Glutathione Synthetase Products

Product Documents for Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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