Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM599

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glycophorin A Antibodies »

Product Specifications

Immunogen

Recombinant human Glycophorin A protein (Uniprot: P02724)

Localization

Cell surface

Marker

Erythrocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Glycophorin A Antibody (SPM599) - IHC-Prediluted

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Glycophorin A Monoclonal Antibody (SPM599)
Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) - IHC-Prediluted [NBP2-48395] - Formalin-fixed, paraffin-embedded human Placenta stained with Glycophorin A Monoclonal Antibody (SPM599)

Applications for Glycophorin A Antibody (SPM599) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Glycophorin A

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Alternate Names

CD235a, GPA, GYPA

Gene Symbol

GYPA

Additional Glycophorin A Products

Product Documents for Glycophorin A Antibody (SPM599) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glycophorin A Antibody (SPM599) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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