Glyoxalase I Antibody (Glo1a)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19015

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Glo1a

Concentration

1 mg/ml

View all Glyoxalase I Antibodies »

Product Specifications

Immunogen

Full length human GLO1 protein [Swiss-Prot #Q04760].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glyoxalase I Antibody (Glo1a)

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015]

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015]

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015]

Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015]

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University.
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015]

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015]

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.
View 2 more images

Applications for Glyoxalase I Antibody (Glo1a)

Application
Recommended Usage

ELISA

0.15 ng / 1 ug protein

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

reported in scientific literature (10.3892/ol.2021.12808)

Immunoprecipitation

reported in scientific literature

Simple Western

1:50
Application Notes
This GLO1 antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 21 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-19015 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glyoxalase I

Glyoxalase 1 (GLO1) is the main enzyme in glyoxalase system, a metabolic pathway responsible for detoxifying alpha-oxoaldehydes, particularly methylglyoxal; and is known for converting reactive dicarbonyls into non-toxic intermediates ultimately protecting living cells from producing and accumulating advanced glycation end products, AGEs. GLO1 has also been studied with reference to anxiety, and a murine model showed that this gene's overexpression increases anxiety-related behavior by reducing the GABAA receptor agonist MG. Moreover, an association analysis in a human population with anxiety disorder subtypes suggested a role for GLO1's implication in this phenotype. GLO1 plays an important role in tumour growth and partly in chemotherapy survival as evidenced by a number of studies suggesting that some tumours rely on GLO1 to increase their viability and resistance to multiple chemotherapeutic agents. Furthermore, genetic studies have implicated GLO1 polymorphisms in panic disorder and restless legs syndrome (RLS).

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody (Glo1a)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glyoxalase I Antibody (Glo1a)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.