Glyoxalase I Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98271
![Western Blot: Glyoxalase I Antibody [NBP2-98271]](https://resources.bio-techne.com/images/products/Glyoxalase-I-Antibody-Western-Blot-NBP2-98271-img0004.jpg "Western Blot: Glyoxalase I Antibody [NBP2-98271]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Glyoxalase I Antibody
Immunogen
Produced in rabbits immunized with purified, recombinant Mouse Glyoxalase I (Accession#: NP_079650.3; Ala 2-Ile 184)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Glyoxalase I Antibody
Western Blot: Glyoxalase I Antibody [NBP2-98271]
Western Blot: Glyoxalase I Antibody [NBP2-98271] - Anti-Glyoxalase I rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271]](https://resources.bio-techne.com/images/products/Glyoxalase-I-Antibody-Immunoprecipitation-NBP2-98271-img0003.jpg "Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271]")
Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271]
Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271] - Mouse Glyoxalase I was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Glyoxalase I rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Glyoxalase I rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDaApplications for Glyoxalase I Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glyoxalase I
Alternate Names
Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase
Gene Symbol
GLO1
Additional Glyoxalase I Products
Product Documents for Glyoxalase I Antibody
Product Specific Notices for Glyoxalase I Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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