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Glypican 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87697

Novus Biologicals, part of Bio-Techne
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NBP1-87697
NBP1-87697-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCPSEFDYNATDHAGKSANEKADSAGVR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glypican 4 Antibody

Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody [NBP1-87697]

Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody [NBP1-87697]

Immunocytochemistry/Immunofluorescence: Glypican 4 Antibody [NBP1-87697] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697]

Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697]

Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human tonsil shows negative positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697]

Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697]

Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human kidney shows strong positivity in cytoplasm granular in cells in tubules.

Applications for Glypican 4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 4

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with avariable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family(GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. Theseproteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. (provided by RefSeq)

Alternate Names

GPC4, K-glypican

Gene Symbol

GPC4

Additional Glypican 4 Products

Product Documents for Glypican 4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glypican 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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