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Glypican 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48723

Novus Biologicals, part of Bio-Techne
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NBP2-48723
NBP2-48723-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRN

Reactivity Notes

Mouse (88%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glypican 5 Antibody

Immunohistochemistry-Paraffin: Glypican 5 Antibody [NBP2-48723]

Immunohistochemistry-Paraffin: Glypican 5 Antibody [NBP2-48723]

Immunohistochemistry-Paraffin: Glypican 5 Antibody [NBP2-48723] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry: Glypican 5 Antibody [NBP2-48723]

Immunohistochemistry: Glypican 5 Antibody [NBP2-48723]

Immunohistochemistry: Glypican 5 Antibody [NBP2-48723] - Staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells, subset of glial cells and neuropil displays strong cytoplasmic staining while endothelial cells are moderately stained.
Immunohistochemistry-Paraffin: Glypican 5 Antibody [NBP2-48723]

Immunohistochemistry-Paraffin: Glypican 5 Antibody [NBP2-48723]

Immunohistochemistry-Paraffin: Glypican 5 Antibody [NBP2-48723] - Staining in human cerebral cortex and pancreas tissues using anti-GPC5 antibody. Corresponding GPC5 RNA-seq data are presented for the same tissues.

Applications for Glypican 5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 5

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Alternate Names

GPC5

Gene Symbol

GPC5

Additional Glypican 5 Products

Product Documents for Glypican 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glypican 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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