GM-CSF Antibody (BVD2-21C11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34570
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Cynomolgus Monkey, Rhesus Macaque
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # BVD2-21C11
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human GM-CSF protein (Uniprot: P04141)
Localization
Secreted (extracellular)
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32967).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for GM-CSF Antibody (BVD2-21C11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Frozen
0.5-1ug/ml
Immunoprecipitation
0.5-1ug/500ug protein lysate
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Neutralization Studies: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Neutralization Studies: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Gene Symbol
CSF2
Additional GM-CSF Products
Product Documents for GM-CSF Antibody (BVD2-21C11) - Azide and BSA Free
Product Specific Notices for GM-CSF Antibody (BVD2-21C11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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