GM130/GOLGA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89756

Novus Biologicals, part of Bio-Techne
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NBP1-89756
NBP1-89756-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GM130/GOLGA2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKE

Marker

Golgi Apparatus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GM130/GOLGA2 Antibody

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756]

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756]

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar protein distribution across tissues to independent antibody NBP1-89758 (B).
Western Blot: GM130/GOLGA2 Antibody [NBP1-89756]

Western Blot: GM130/GOLGA2 Antibody [NBP1-89756]

Western Blot: GM130/GOLGA2 Antibody [NBP1-89756] - Analysis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar pattern to independent antibody NBP1-89758 (B).
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.
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Applications for GM130/GOLGA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GM130/GOLGA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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