Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed for 10 min using 10% formalin and then permeabilized for 5 min using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GOLGA2 at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: GM130/GOLGA2 AntibodyBSA Free [NBP2-53420]

Western Blot: GM130/GOLGA2 Antibody [NBP2-53420] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-GOLGA2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP2-53420] - Analysis of a FFPE tissue section of mouse intesting using GM130/GOLGA2 antibody at 1:1500. The antibody generated staining in the the Golgi apparatus in the perinuclear region.

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to Biotin (NBP2-53420B) at 5 ug/ml for 1 hour at room temperature and detected with Streptavidin conjugated to DyLight 488. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on NIH3T3 cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 min at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on MCF7 cells with GM130/GOLGA2 Antibody NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunohistochemistry: Rabbit Polyclonal GM130/GOLGA2 Antibody [NBP2-53420] -

Immunohistochemistry: Rabbit Polyclonal GM130/GOLGA2 Antibody [NBP2-53420] - Staining of mouse C57BL/6 retina using GM130/GOLGA2 Antibody. GOLGA (red), BS-1 lectin (green), DAPI (blue). Primary antibody dilution: 1:200. Image from a verified customer review.

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Applications for GM130/GOLGA2 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-5 ug/mL

Immunohistochemistry

1:1000 - 1:1500

Immunohistochemistry-Paraffin

1:1000 - 1:1500

Western Blot

0.5 ug/mL

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-53420 in the following applications:

IF- fixed tissue (1 Review)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for GM130/GOLGA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

GM130/GOLGA2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53420

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all GM130/GOLGA2 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for GM130/GOLGA2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Western Blot: GM130/GOLGA2 AntibodyBSA Free [NBP2-53420]

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunohistochemistry: Rabbit Polyclonal GM130/GOLGA2 Antibody [NBP2-53420] -

Applications for GM130/GOLGA2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: GM130/GOLGA2

Long Name

Alternate Names

Gene Symbol

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for GM130/GOLGA2 Antibody - BSA Free