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GMDS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33424

Novus Biologicals, part of Bio-Techne
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NBP1-33424

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (97%), Mouse (99%), Rat (98%), Rhesus Macaque (99%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GMDS. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%), Zebrafish (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GMDS Antibody

Knockdown Validated: GMDS Antibody [NBP1-33424]

Knockdown Validated: GMDS Antibody [NBP1-33424]

Knockdown Validated: GMDS Antibody [NBP1-33424] - a GMDS mRNA level in A549 cells and H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by quantitative real-time PCR (normalized to GAPDH mRNA). Data shown here was one out of three independent experiments (**p < 0.01). b Relative GMDS protein level in A549 cells and H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by western blot. GAPDH protein was used as internal control. c-d. Representative microscope pictures of A549 cells. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1) licensed under a CC-BY license.
Immunohistochemistry: GMDS Antibody [NBP1-33424]

Immunohistochemistry: GMDS Antibody [NBP1-33424]

Immunohistochemistry: GMDS Antibody [NBP1-33424] - Representative images of GMDS IHC staining in human lung adenocarcinoma and adjacent normal tissues. (Magnification, left is 20x, right is 100x). Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1) licensed under a CC-BY license.
Western Blot: GMDS Antibody [NBP1-33424]

Western Blot: GMDS Antibody [NBP1-33424]

Western Blot: GMDS Antibody [NBP1-33424] - GMDS protein level in different cell lines including BEAS-2B (1), MRC-5 (2), HEK-293 (3), A549 (4), H1299 (5), SPC-A-1 (6). A59, H1299 and SPC-A-1 are all human lung adenocarcinoma cell lines. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1) licensed under a CC-BY license.

Applications for GMDS Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GMDS

GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose,the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third stepsof the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX inhumans (MIM 137020).(supplied by OMIM)

Alternate Names

EC 4.2.1.47, GDP-D-mannose dehydratase, GDP-mannose 4,6-dehydratase, GMDGDP-mannose 4,6 dehydratase, SDR3E1, short chain dehydrogenase/reductase family 3E, member 1

Entrez Gene IDs

2762 (Human)

Gene Symbol

GMDS

UniProt

Additional GMDS Products

Product Documents for GMDS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GMDS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.