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GMF-beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89755

Novus Biologicals, part of Bio-Techne
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NBP1-89755
NBP1-89755-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SESLVVCDVAEDLVEKLRKFRFRKETNNAAIIMKIDKDKRLVVLDEELEGISPDELKDELPERQPRFIVYSYKYQHDDGRVSYPLCFIFSSPVGCKPEQQMMYAGSKN

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26101216).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GMF-beta Antibody

Western Blot: GMF-beta Antibody [NBP1-89755]

Western Blot: GMF-beta Antibody [NBP1-89755]

Western Blot: GMF-beta Antibody [NBP1-89755] - Tubulogenesis of human U87 glioblastoma cells is inhibited by GMF-beta knockdown. Protein levels of GMF-beta in human glial cell line (HEB) and human glioma cell lines of the different grades (CHG5, SHG44, U87). Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/5509), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: GMF-beta Antibody [NBP1-89755]

Immunocytochemistry/ Immunofluorescence: GMF-beta Antibody [NBP1-89755]

Immunocytochemistry/Immunofluorescence: GMF-beta Antibody [NBP1-89755] - GMF-beta is involved in neovasculogenesis in human glioblastoma. A. Co-expression of GMF-beta and CD31 in several tumor cells. B. The GMF-beta+/CD31+ incomplete microvessel-like structure in hypovascular zones. C. GMF-beta+/CD31+ immature microvessel in hypovascular zones. D. Single GMF-beta+/CD31+ mature microvessel in vascularized areas. E. GMF-beta-/CD31+ mature microvessels in vascularized areas. Red arrows denote GMF-beta staining, black arrows indicate CD31 staining. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/5509), licensed under a CC-BY license.
Immunohistochemistry: GMF-beta Antibody [NBP1-89755]

Immunohistochemistry: GMF-beta Antibody [NBP1-89755]

Immunohistochemistry: GMF-beta Antibody [NBP1-89755] - GMF-beta expression is positively associated with tumor grade and microvessel density (MVD) in human glioma. Distributions of GMF-beta in normal brain tissue (left panel), low-grade (middle panel) and high-grade glioma (right panel). Solid arrows, GMF-beta staining (brown); open arrows, negative staining; Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/5509), licensed under a CC-BY license.

Applications for GMF-beta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GMF-beta

This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition ofproliferation of tumor cells

Long Name

Glia Maturation Factor beta

Alternate Names

GMFB, GMFbeta

Entrez Gene IDs

2764 (Human); 63985 (Mouse)

Gene Symbol

GMFB

UniProt

Additional GMF-beta Products

Product Documents for GMF-beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GMF-beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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