GMF-beta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89755
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SESLVVCDVAEDLVEKLRKFRFRKETNNAAIIMKIDKDKRLVVLDEELEGISPDELKDELPERQPRFIVYSYKYQHDDGRVSYPLCFIFSSPVGCKPEQQMMYAGSKN
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26101216).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GMF-beta Antibody
Western Blot: GMF-beta Antibody [NBP1-89755]
Western Blot: GMF-beta Antibody [NBP1-89755] - Tubulogenesis of human U87 glioblastoma cells is inhibited by GMF-beta knockdown. Protein levels of GMF-beta in human glial cell line (HEB) and human glioma cell lines of the different grades (CHG5, SHG44, U87). Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/5509), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: GMF-beta Antibody [NBP1-89755]
Immunocytochemistry/Immunofluorescence: GMF-beta Antibody [NBP1-89755] - GMF-beta is involved in neovasculogenesis in human glioblastoma. A. Co-expression of GMF-beta and CD31 in several tumor cells. B. The GMF-beta+/CD31+ incomplete microvessel-like structure in hypovascular zones. C. GMF-beta+/CD31+ immature microvessel in hypovascular zones. D. Single GMF-beta+/CD31+ mature microvessel in vascularized areas. E. GMF-beta-/CD31+ mature microvessels in vascularized areas. Red arrows denote GMF-beta staining, black arrows indicate CD31 staining. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/5509), licensed under a CC-BY license.Immunohistochemistry: GMF-beta Antibody [NBP1-89755]
Immunohistochemistry: GMF-beta Antibody [NBP1-89755] - GMF-beta expression is positively associated with tumor grade and microvessel density (MVD) in human glioma. Distributions of GMF-beta in normal brain tissue (left panel), low-grade (middle panel) and high-grade glioma (right panel). Solid arrows, GMF-beta staining (brown); open arrows, negative staining; Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/5509), licensed under a CC-BY license.Applications for GMF-beta Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GMF-beta
Long Name
Glia Maturation Factor beta
Alternate Names
GMFB, GMFbeta
Gene Symbol
GMFB
UniProt
Additional GMF-beta Products
Product Documents for GMF-beta Antibody
Product Specific Notices for GMF-beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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