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GNAI3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-14246

Novus Biologicals, part of Bio-Techne
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NB120-14246
NB120-14246-0.025mg

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (100%), Guinea Pig (100%), Hamster (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

KNNLKECGLY, corresponding to amino acids 345-354 of GAI3.

Localization

Cell Membrane

Specificity

recognises GNAI3.

Clonality

Polyclonal

Host

Chicken

Applications for GNAI3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200 - 1:500

Western Blot

1:200 - 1:500
Application Notes
ICC: Use at a dilution of 1/200. Western Blot: Use at a dilution of 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). tissue and cell immunostaining: Use at a starting dilution of 1/500. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GNAI3

GAI3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor regulated K(+) channels.

Alternate Names

FLJ26559, G(i) alpha-3, guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6, guanine nucleotide-binding protein G(k) subunit alpha

Entrez Gene IDs

2773 (Human); 56470 (Mouse); 59293 (Rat)

Gene Symbol

GNAI3

Additional GNAI3 Products

Product Documents for GNAI3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GNAI3 Antibody - Azide and BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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