GNAI3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32617

Novus Biologicals, part of Bio-Techne
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NBP1-32617
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Chicken (97%), Guinea Pig (97%), Porcine (99%), Rat (97%), Rhesus Macaque (100%), Xenopus (94%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GNAI3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAI3. The exact sequence is proprietary.

Reactivity Notes

Japanese Medaka (91%), Xenopus laevis (94%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GNAI3 Antibody

Knockout Validated: GNAI3 Antibody [NBP1-32617]

Knockout Validated: GNAI3 Antibody [NBP1-32617]

Knockout Validated: GNAI3 Antibody [NBP1-32617] - Wild-type (WT) and G protein alpha Inhibitor 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha Inhibitor 3 antibody [N2C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: GNAI3 Antibody [NBP1-32617]

Western Blot: GNAI3 Antibody [NBP1-32617]

Western Blot: GNAI3 Antibody [NBP1-32617] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: GNAI3 Antibody [NBP1-32617]

Immunohistochemistry-Paraffin: GNAI3 Antibody [NBP1-32617]

Immunohistochemistry-Paraffin: GNAI3 Antibody [NBP1-32617] - SAS xenograft, using G protein alpha Inhibitor 3 antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for GNAI3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GNAI3

GAI3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor regulated K(+) channels.

Alternate Names

FLJ26559, G(i) alpha-3, guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6, guanine nucleotide-binding protein G(k) subunit alpha

Gene Symbol

GNAI3

Additional GNAI3 Products

Product Documents for GNAI3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GNAI3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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