GNAT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20926

Novus Biologicals, part of Bio-Techne
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NBP1-20926
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GNAT3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KNQFLDLNLKKEDKE corresponding to internal region according to NP_001095856.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GNAT3 Antibody

Immunohistochemistry-Paraffin: GNAT3 Antibody [NBP1-20926]

Immunohistochemistry-Paraffin: GNAT3 Antibody [NBP1-20926]

Immunohistochemistry-Paraffin: GNAT3 Antibody [NBP1-20926] - (3.8ug/ml) staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GNAT3 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 27 kDa band in human colon and testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4 kDa band according to NP_001095856.1. The 27 kDa band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GNAT3

Function: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonuim and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.; Subcellular location: cytoplasm; Tissue specificity: Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and fungiform papillae of the tongue as well as in palatal taste buds at protein level. Expressed in enteroendocrine cells of the gut, such as in subsets of enteroendocrine cells in the midjejunum and brush cells. Detected also in spermatozoa.

Alternate Names

GDCA, guanine nucleotide binding protein, alpha transducing 3, guanine nucleotide-binding protein G(t) subunit alpha-3, gustducin, Gustducin alpha-3 chain

Entrez Gene IDs

346562 (Human); 242851 (Mouse); 286924 (Rat)

Gene Symbol

GNAT3

UniProt

A8MTJ3 (Human)

Additional GNAT3 Products

Product Documents for GNAT3 Antibody

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Product Specific Notices for GNAT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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