GNB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3433

Novus Biologicals, part of Bio-Techne
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NB120-3433
NB120-3433-0.025mg
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Key Product Details

Species Reactivity

Human, Mouse, Bovine, Drosophila, Sheep

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all GNB1 Antibodies »

Product Specifications

Immunogen

Synthetic Peptide: R(8) Q E A E Q L K N Q I R D A R K A C(25)

Reactivity Notes

Expected to cross-react with Rat and Amphibians due to sequence identity.

Specificity

The NB120-3433 immunizing peptide corresponds to amino acid residues 8-25 from human Tr beta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GNB1 Antibody

Western Blot: GNB1 Antibody [NB120-3433]

Western Blot: GNB1 Antibody [NB120-3433]

Western Blot: GNB1 Antibody [NB120-3433] - Analysis of Tr beta on sheep retinal extracts.

Applications for GNB1 Antibody

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
WB: Detects an approx. 37 kDa protein representing Transducin beta from sheep retinal extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GNB1

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

Alternate Names

beta subunit, signal-transducing proteins GS/GI, G protein, beta-1 subunit, guanine nucleotide binding protein (G protein), beta polypeptide 1, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1

Entrez Gene IDs

2782 (Human)

Gene Symbol

GNB1

Additional GNB1 Products

Product Documents for GNB1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GNB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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