GNB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16699

Novus Biologicals, part of Bio-Techne
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NBP2-16699
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (100%), Canine (100%), Chicken (96%), Guinea Pig (100%), Rhesus Macaque (100%), Xenopus (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GNB1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (96%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GNB1 Antibody

Western Blot: GNB1 Antibody [NBP2-16699]

Western Blot: GNB1 Antibody [NBP2-16699]

Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: GNB1 Antibody [NBP2-16699]

Immunocytochemistry/ Immunofluorescence: GNB1 Antibody [NBP2-16699]

Immunocytochemistry/Immunofluorescence: GNB1 Antibody [NBP2-16699] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 protein stained by GNB1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [9810] diluted at 1:20000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699]

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699]

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Mouse testis. GNB1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for GNB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Proximity Ligation Assay

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GNB1

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]

Alternate Names

beta subunit, signal-transducing proteins GS/GI, G protein, beta-1 subunit, guanine nucleotide binding protein (G protein), beta polypeptide 1, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1

Gene Symbol

GNB1

Additional GNB1 Products

Product Documents for GNB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GNB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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