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GOLGA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2442

Novus Biologicals, part of Bio-Techne
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NB100-2442

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-HSQSRASKEGPGE corresponding to C-Terminus according to NP_005886.2.

Localization

Cytoplasmic and Golgi apparatus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GOLGA3 Antibody

Western Blot: GOLGA3 Antibody [NB100-2442]

Western Blot: GOLGA3 Antibody [NB100-2442]

Western Blot: GOLGA3 Antibody [NB100-2442] - Staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.1ug/ml)

Applications for GOLGA3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in human HeLa lysates (calculated MW of 167 kDa band according to NP_005886.2). An additional band of unknown identity was also consistently observed at approx. 25 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GOLGA3

GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.

Alternate Names

GCP170Golgi peripheral membrane protein, golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi complex-associated protein of 170 kDa, Golgi membrane associated protein, golgin A3, Golgin subfamily A member 3, golgin-160, golgin-165, male enhanced antigen-2, MEA-2, SY2/SY10 protein

Entrez Gene IDs

2802 (Human)

Gene Symbol

GOLGA3

Additional GOLGA3 Products

Product Documents for GOLGA3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GOLGA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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