GOLGB1/Giantin Antibody

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Untreated MDA-MB-231 cells showed perinuclear colonization of Giantin and other proteins, demonstrating this cell line exhibits the architecture of a typical Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. Secondary antibodies in other channels. Nuclei were stained with DAPI (gray channel). Fluorescence microscopy was used to examine stained cells. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Effect of ActD on the perinuclear localization of the Golgi apparatus of MDA-MB-231 cells assessed by immunofluorescence to Giantin, GM130 and TGN46. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Shows the combination effects of antitumor drugs and ARF1 disruptors on the MDA-MB-231 cells Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.

Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321]

Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.