Golgin 97 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38528

Novus Biologicals, part of Bio-Techne
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NBP2-38528
NBP2-38528-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Golgin 97 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TGAESKITALEQKEQELQALIQQLSIDLQKVTAETQEKEDVITHLQEKVASLEKRLEQNLSGEEHLQELLK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Golgin 97 Antibody

Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528]

Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528]

Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Analysis in human testis and skeletal muscle tissues. Corresponding GOLGA1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Golgin 97 Antibody [NBP2-38528]

Immunocytochemistry/ Immunofluorescence: Golgin 97 Antibody [NBP2-38528]

Immunocytochemistry/Immunofluorescence: Golgin 97 Antibody [NBP2-38528] - Staining of human cell line A549 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528]

Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528]

Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells.
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Applications for Golgin 97 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Golgin 97

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Alternate Names

gap junction protein, alpha 4, 37kD, golgi autoantigen, golgin subfamily a, 1, golgin A1, Golgin subfamily A member 1, Golgin-97, MGC33154

Gene Symbol

GOLGA1

UniProt

Q92805 (Human)

Additional Golgin 97 Products

Product Documents for Golgin 97 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Golgin 97 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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